[Cloud-Clone 한국공식대리점] ELISA Kit for Pepsin (PP)

[Cloud-Clone 한국공식대리점] ELISA Kit for Pepsin (PP)

[ELISA Kit for Pepsin (PP)]

Product Information:

• Product No.: CEA632Hu
• Organism Species: Homo sapiens (Human) Same name, Different species.
• Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Test Method: Competitive Inhibition
• Assay Length: 2h
• Detection Range: 2.47-200ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.87ng/mL.
• UOM: 48T, 96T, 96T*5, 96T*10, 96T*100

This assay has high sensitivity and excellent specificity for detection of Pepsin (PP).
No significant cross-reactivity or interference between Pepsin (PP) and analogues was observed.

Matrices listed below were spiked with certain level of recombinant Pepsin (PP) and the recovery rates were calculated by comparing the measured value to the expected amount of Pepsin (PP) in samples.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pepsin (PP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pepsin (PP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pepsin (PP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.